| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.145673 |
| Chromosome: | chromosome 5 |
| Location: | 1971406 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241639 | (1 of 3) K09858 - SEC-C motif domain protein (K09858) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACACACGCCCCAACAGAATGACGAGG |
| Internal bar code: | TCGGAGCCAGAAATCGTCAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 166 |
| LEAP-Seq percent confirming: | 99.604 |
| LEAP-Seq n confirming: | 503 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTTTGTGGAGGACGTGT |
| Suggested primer 2: | CCCACAACTAGGGGCTAACA |