| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.145744 |
| Chromosome: | chromosome 7 |
| Location: | 5076108 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347600 | (1 of 1) PTHR11059:SF0 - DNA REPAIR PROTEIN RECN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAGCAGCCGCCGCAGCTCGCGCTCCTCC |
| Internal bar code: | GCTATTGGTACGTCGCAGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 174 |
| LEAP-Seq percent confirming: | 99.7662 |
| LEAP-Seq n confirming: | 3413 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATCCTAAGGGTGTTGTT |
| Suggested primer 2: | AGAACAACATCGGATCTCGC |