Insertion junction: LMJ.RY0402.145761_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.145761
Chromosome:chromosome 14
Location:2195272
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g622700 UMAMIT (Usually Multiple Acids Move In and Out Transporters) type transporter; (1 of 5) PTHR11132//PTHR11132:SF101 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGATAAATCAGTGGCACGTCTTGATGAAT
Internal bar code:AATTGCAGCAACTGCCCACTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:778
LEAP-Seq percent confirming:98.9995
LEAP-Seq n confirming:5937
LEAP-Seq n nonconfirming:60
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:ACCATGATCGAGTTCTTCGG
Suggested primer 2:GCTTACTTTGCGCCTATTGC