| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145777 |
| Chromosome: | chromosome_9 |
| Location: | 2210524 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g392900 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGGTCGCCCATGCCTGGCGCTGCCCAGCC |
| Internal bar code: | GGAGTCGTCTCACCACGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 249 |
| LEAP-Seq percent confirming: | 78.5444 |
| LEAP-Seq n confirming: | 2903 |
| LEAP-Seq n nonconfirming: | 793 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAAAAGAAACTCGCCAGC |
| Suggested primer 2: | ACAACCTGAATATGGCCAGC |