Insertion junction: LMJ.RY0402.145791_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.145791
Chromosome:chromosome 10
Location:4854852
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g454300 (1 of 1) IPR003613//IPR011009 - U box domain // Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGACCGGACAAACTGGCCAGCTTGATTG
Internal bar code:TCTTCTATGCCATCTGTCAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:993
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTGTCTTTTGCCGACTA
Suggested primer 2:GCCAGCTTCTGCCTCATAAC