| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.145797 |
| Chromosome: | chromosome 6 |
| Location: | 1185203 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257450 | ATPvL1,ATPVL1 | (1 of 1) K02155 - V-type H+-transporting ATPase 16kDa proteolipid subunit (ATPeV0C, ATP6L); Vacuolar ATP synthase subunit C proteolipid | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACAACTAGCCCAAATATGCTGCTGGTAT |
| Internal bar code: | TTTGTCAATAGATATAAGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 800 |
| LEAP-Seq percent confirming: | 99.6762 |
| LEAP-Seq n confirming: | 8926 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTTATCTGCCACTGCCT |
| Suggested primer 2: | TGCGTAGACTTACCGCACAC |