Insertion junction: LMJ.RY0402.145807_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.145807
Chromosome:chromosome 1
Location:3442974
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g021950 (1 of 1) PF08393//PF12777//PF12781 - Dynein heavy chain, N-terminal region 2 (DHC_N2) // Microtubule-binding stalk of dynein motor (MT) // ATP-binding dynein motor region D5 (AAA_9) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGCGGGTGACGGGGTGACAGCGCGGCGG
Internal bar code:AGGTGGCTTTAAATATGCCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:136
LEAP-Seq percent confirming:99.5546
LEAP-Seq n confirming:894
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCGCTCTCTGTGATATGC
Suggested primer 2:GCTTCTTGACTCATCTGCCC