| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.145809 |
| Chromosome: | chromosome_6 |
| Location: | 786755 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g254100 | HRP6 | sense | 5'UTR_intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCAGTTCCAGTCCCTCGCTGCTGTTAAGG |
| Internal bar code: | TCGGTAAGTCTGTCCCGGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 158 |
| LEAP-Seq percent confirming: | 5.65782 |
| LEAP-Seq n confirming: | 209 |
| LEAP-Seq n nonconfirming: | 3485 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGACTAGACACTGCGGA |
| Suggested primer 2: | CTGCAAGTTCTCCAGGAAGG |