Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.145824 |
Chromosome: | chromosome_6 |
Location: | 4067643 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g278212 | CGL46 | Predicted protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCATGTTAGGCCCGCGCATGCCGCTTGTC |
Internal bar code: | GTTTTTCGACAGTTTGCATGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 36 |
LEAP-Seq percent confirming: | 97.2789 |
LEAP-Seq n confirming: | 143 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGGAAAGCTACAGTTCCTG |
Suggested primer 2: | GACAGGTGCTGATGGTGATG |