Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.145826 |
Chromosome: | chromosome_5 |
Location: | 2202047 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g234652 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCTTTACTGTAAACTGCACCGCGCTTTGC |
Internal bar code: | GTTAAATGGCACGGGCTCTCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 371 |
LEAP-Seq percent confirming: | 99.3007 |
LEAP-Seq n confirming: | 284 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTATGTTTGCTGTTGGGGCT |
Suggested primer 2: | TGTTACATACCAGGCGGTCA |