| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145830 |
| Chromosome: | chromosome 6 |
| Location: | 5146290 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281350 | LON1 | (1 of 1) K08675 - Lon-like ATP-dependent protease [EC:3.4.21.-] (PRSS15, PIM1); Mitochondrial LON protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCTCCCCGAACCCCATCTCCCTTCCTC |
| Internal bar code: | GTGGGCTTTGGAGATTGACACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 99.8077 |
| LEAP-Seq n confirming: | 519 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGCGAGGTAGACAGCA |
| Suggested primer 2: | CGTTCAGGGGGAACGTAGTA |