| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145835 |
| Chromosome: | chromosome 10 |
| Location: | 4801319 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453850 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTACGAAAGCCGCCTCGCCACACGCGCTG |
| Internal bar code: | CGAGACCGTCAGGCTCAAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 99.7708 |
| LEAP-Seq n confirming: | 1306 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGAACTCAGGGACTGGAG |
| Suggested primer 2: | GCACGGTCTCCTACAAGCTC |