| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.145844 |
| Chromosome: | chromosome 6 |
| Location: | 8184122 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305350 | (1 of 1) PTHR11319//PTHR11319:SF32 - G PROTEIN-COUPLED RECEPTOR-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTGGCACGGTGCGTGTGCGTGCGCGTG |
| Internal bar code: | AAATGCGCTTCATCAAGAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 359 |
| LEAP-Seq percent confirming: | 79.1726 |
| LEAP-Seq n confirming: | 2794 |
| LEAP-Seq n nonconfirming: | 735 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCTACATGGGTGAGTTG |
| Suggested primer 2: | GGTACTTGCTGTACGCCTCC |