| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.145845 |
| Chromosome: | chromosome 8 |
| Location: | 1495865 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364751 | BLZ16 | bZIP transcription factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCCCAAGCCCAAGCCCAAGCAACGAGA |
| Internal bar code: | CTTGAATTAATTGGCTGAGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 57 |
| LEAP-Seq percent confirming: | 92.0635 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGCTCTGAGGATTCTATG |
| Suggested primer 2: | AGATGTCCAGGAAAACGGTG |