| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.145875 |
| Chromosome: | chromosome 10 |
| Location: | 1718278 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430501 | (1 of 1) PTHR22904//PTHR22904:SF331 - TPR REPEAT CONTAINING PROTEIN // CARBOXYLATE CLAMP-TETRATRICOPEPTIDE REPEAT PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTCGCGCTCCTCACCCTGCTGCTGCGC |
| Internal bar code: | TCGGTCCTTGGCATGGCTCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 853 |
| LEAP-Seq percent confirming: | 83.7143 |
| LEAP-Seq n confirming: | 3516 |
| LEAP-Seq n nonconfirming: | 684 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCGCCTTAGCAGTTAGCA |
| Suggested primer 2: | ACTATCACGCCCCTTGACAC |