| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145883 |
| Chromosome: | chromosome 3 |
| Location: | 2512132 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159900 | (1 of 1) K07179 - RIO kinase 2 (RIOK2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCAGGTTGAACTCGTTGAAGTCGCAGT |
| Internal bar code: | TGTTGCCACGACTCTGACGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 196 |
| LEAP-Seq percent confirming: | 39.7135 |
| LEAP-Seq n confirming: | 998 |
| LEAP-Seq n nonconfirming: | 1515 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCGAACAGCACACAGGT |
| Suggested primer 2: | GCAGCATTAAAGGAGTTCGC |