| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.145883 |
| Chromosome: | chromosome 6 |
| Location: | 8444771 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307300 | ANT2 | ADP/ATP carrier protein, mitochondrial; (1 of 3) K05863 - solute carrier family 25 (mitochondrial adenine nucleotide translocator), member 4/5/6/31 (SLC25A4S, ANT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTACTTCGGGCTGTACGACACCGCGGT |
| Internal bar code: | ATTAACAGAGCGGACGAGTTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 260 |
| LEAP-Seq percent confirming: | 99.3343 |
| LEAP-Seq n confirming: | 9401 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTCGGAGTGGGTCACAT |
| Suggested primer 2: | AAATCAATACCCATGCCCAA |