Insertion junction: LMJ.RY0402.145885_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.145885
Chromosome:chromosome 3
Location:7356565
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g206350 OPR19 OctotricoPeptide Repeat protein 19; (1 of 3) PF06743 - FAST kinase-like protein, subdomain 1 (FAST_1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGTCATCCTCAAAGGCAGCGTAGTGCTC
Internal bar code:CCCAAACCCACGATCTTCGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:343
LEAP-Seq percent confirming:99.789
LEAP-Seq n confirming:473
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGTAGGGCTTGCCGACTG
Suggested primer 2:CCGGTCACAGTATTCCGACT