| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.145927 |
| Chromosome: | chromosome 4 |
| Location: | 2728791 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223500 | (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCAAAGCCCCCGTGCCTCATCCGCCCG |
| Internal bar code: | CAAACGGATTGGAACTCTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1113 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTATGGCCTCATCGCTTC |
| Suggested primer 2: | ATGTTGGCATGGTGACCTTT |