| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145932 |
| Chromosome: | chromosome 12 |
| Location: | 5586558 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531850 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTGCCAAGCATGGCAGCCGTGCCAGGCG |
| Internal bar code: | GAGCTACGACTGGCGCAGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 352 |
| LEAP-Seq percent confirming: | 93.6451 |
| LEAP-Seq n confirming: | 781 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCGGATACACATGGACT |
| Suggested primer 2: | TAGCATGACTTCAGCAACCG |