| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.145941 |
| Chromosome: | chromosome 6 |
| Location: | 7319248 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298800 | ARS73A,ARS73a,LTH5 | Arylsulfatase 73a | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCTTATGGTTTCTGGCTGCCGATGCTG |
| Internal bar code: | GCTGGAAGTCTGTCACCGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 282 |
| LEAP-Seq percent confirming: | 99.7057 |
| LEAP-Seq n confirming: | 6776 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAATCCTCGGTGCTGTGGT |
| Suggested primer 2: | CTTTGCCTCTGTAGGTTGGC |