| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145968 |
| Chromosome: | chromosome 1 |
| Location: | 7109588 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g051350 | (1 of 3) PF13563 - 2'-5' RNA ligase superfamily (2_5_RNA_ligase2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTCACACATGGTTCCCCTGCACCAGCAC |
| Internal bar code: | GCGGCAAGAAGAACGGGGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 99.8994 |
| LEAP-Seq n confirming: | 2980 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGTAGCGGAATGTGTCC |
| Suggested primer 2: | CATACAGCATTCATCGCCAC |