| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.145968 |
| Chromosome: | chromosome 1 |
| Location: | 7109591 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g051350 | (1 of 3) PF13563 - 2'-5' RNA ligase superfamily (2_5_RNA_ligase2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAAGGCGCAGACAACAACCGGGGGCGT |
| Internal bar code: | TGAGCGCTGGCAGCAGCTGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 908 |
| LEAP-Seq percent confirming: | 99.6217 |
| LEAP-Seq n confirming: | 12902 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGTAGCGGAATGTGTCC |
| Suggested primer 2: | CATACAGCATTCATCGCCAC |