| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146007 |
| Chromosome: | chromosome 3 |
| Location: | 6667056 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197250 | (1 of 1) PTHR13948//PTHR13948:SF25 - RNA-BINDING PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGCTCGCGGTCCACTTCGAGTCCCTGC |
| Internal bar code: | GATGCGCGGAACAAGCAGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 492 |
| LEAP-Seq percent confirming: | 99.9648 |
| LEAP-Seq n confirming: | 2842 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGGTCGCTGGGTATGAA |
| Suggested primer 2: | TGACGACGTGGCTGATCTAC |