| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146008 |
| Chromosome: | chromosome 12 |
| Location: | 4293432 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519651 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCTACTGTACTGACCTACCATGGGTTTA |
| Internal bar code: | TGAATCAGGCTGCGGCAATCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 98.9967 |
| LEAP-Seq n confirming: | 2368 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACACGTTCAACTCAAGC |
| Suggested primer 2: | TGCTTGTTAGGTGACGTTGC |