| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146017 |
| Chromosome: | chromosome 1 |
| Location: | 4824459 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033450 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCAACGATGTGGCTTTCAGGCTCCGGC |
| Internal bar code: | TCGACCCATGAGCGACGAGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 735 |
| LEAP-Seq percent confirming: | 99.7449 |
| LEAP-Seq n confirming: | 5864 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGGGTAGAAGTGGAGGG |
| Suggested primer 2: | GTGTCTGCCCAGTGAGTCAA |