| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.146063 |
| Chromosome: | chromosome 17 |
| Location: | 3543242 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725376 | (1 of 1) IPR000104//IPR001859//IPR009148 - Antifreeze protein, type I // Trypanosoma cruzi ribosomal protein P2-like // Streptococcal non-M secreted SibA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTGGGGCATACCAGATAGCAGAGACGTG |
| Internal bar code: | GAGCCCGTATCGGAGAGCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 98.5714 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGAGATCAGACGGGCTC |
| Suggested primer 2: | CCTTCTCCTACTCCACAGCG |