Insertion junction: LMJ.RY0402.146067_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.146067
Chromosome:chromosome 2
Location:2348192
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g090850 CLPB3 (1 of 1) PTHR11638//PTHR11638:SF121 - ATP-DEPENDENT CLP PROTEASE // CHAPERONE PROTEIN CLPB3, CHLOROPLASTIC; ClpB chaperone, Hsp100 family 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTAACATCAGCAGATGTATCTACCCCAAT
Internal bar code:CATGTTGCTTCGCGCTCCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:773
LEAP-Seq percent confirming:88.1346
LEAP-Seq n confirming:7309
LEAP-Seq n nonconfirming:984
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGATGAGGTGGCCGAAAA
Suggested primer 2:GTCTGGATCTGACGGAGAGC