| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146078 |
| Chromosome: | chromosome 5 |
| Location: | 1381653 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244300 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGCGTGTGACGAGTTGGCGCGCGGCG |
| Internal bar code: | TAATGGTTAGTCCCATGGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 98.7664 |
| LEAP-Seq n confirming: | 1281 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTTGCATGTATGTTCGC |
| Suggested primer 2: | GGGCGCTGAGAAAAGTAGTG |