| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146080 |
| Chromosome: | chromosome 16 |
| Location: | 4029718 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670200 | Microtubule-associated protein cysteine-rich PDZ-binding protein; (1 of 2) PTHR11805:SF1 - CYSTEINE-RICH PDZ-BINDING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTAACATTTGAATTCGCAACTTGCGCT |
| Internal bar code: | GCGCTGCTGGGGGTGCGTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 873 |
| LEAP-Seq percent confirming: | 89.7558 |
| LEAP-Seq n confirming: | 3014 |
| LEAP-Seq n nonconfirming: | 344 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTAGACAGCAGCAGTGCG |
| Suggested primer 2: | AATGCAAGACGTGCAAACAG |