| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.146086 |
| Chromosome: | chromosome 17 |
| Location: | 4907152 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734500 | ATPvE,ATPVE | Vacuolar ATP synthase subunit E | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGATGATCTCCTTTGTAACTCGGGTTTC |
| Internal bar code: | ATACACTACAGCTTGGACTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 219 |
| LEAP-Seq percent confirming: | 98.8506 |
| LEAP-Seq n confirming: | 86 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGAAGTGTGCGTGTTTGC |
| Suggested primer 2: | GCGTTGGGCACACTATTCTT |