Insertion junction: LMJ.RY0402.146098_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.146098
Chromosome:chromosome 14
Location:2651779
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g626400 CYP33,CYP743C1 (1 of 3) 1.14.14.1//5.3.99.5 - Unspecific monooxygenase / Xenobiotic monooxygenase // Thromboxane-A synthase / Thromboxane synthetase; Cytochrome P450, CYP197 superfamily 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTGGCGAGGTTGGGGGGTGAAGCGCGGT
Internal bar code:GCATTCCGCGACAAACCGTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:525
LEAP-Seq percent confirming:97.2017
LEAP-Seq n confirming:1841
LEAP-Seq n nonconfirming:53
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CCATGGGGTTAGAGGAGTGA
Suggested primer 2:GGTTGATAGGAGGCGTGTGT