| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.146105 |
| Chromosome: | chromosome 4 |
| Location: | 2313225 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCTCCGCGACCCCCTCCACCATGGTTC |
| Internal bar code: | CTTTGGATGGTGATACTGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 65 |
| LEAP-Seq percent confirming: | 84.0399 |
| LEAP-Seq n confirming: | 337 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGAAGTGAGAGGAGGGAG |
| Suggested primer 2: | AGTTGGTGTGAGGAGTTGGG |