| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146107 |
| Chromosome: | chromosome 7 |
| Location: | 1289439 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321900 | (1 of 6) 3.1.26.11 - Ribonuclease Z / tRNase Z | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCGCAGCCGCAACTCACTTTCCATCCA |
| Internal bar code: | GAAATATGGTGAGCCGCGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 589 |
| LEAP-Seq percent confirming: | 99.7526 |
| LEAP-Seq n confirming: | 2419 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGAACGTGATGGGAAGTT |
| Suggested primer 2: | GGTTATGTGTGTCACCGCAG |