Insertion junction: LMJ.RY0402.146112_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.146112
Chromosome:chromosome 13
Location:3742985
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTCTGGATCCAGGCAACCGCCTCCTTCT
Internal bar code:CAAGGATGGTACAGTCGGCCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:243
LEAP-Seq percent confirming:98.6726
LEAP-Seq n confirming:1115
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGCAAGGAGATCTGGTTTGT
Suggested primer 2:CAGGCTCAGAGCTAACCACC