| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146137 |
| Chromosome: | chromosome 13 |
| Location: | 3107044 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g584850 | (1 of 15) 2.5.1.18 - Glutathione transferase / S-(hydroxyalkyl)glutathione lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGCGATAGGTGCACACGCGGCTAGTTA |
| Internal bar code: | GTAGCGTTCAACCTGGCAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 99.7738 |
| LEAP-Seq n confirming: | 3087 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTAACCGAGCCAAACAAC |
| Suggested primer 2: | GGCAAGATTGCTGAGTGTGA |