Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.146141 |
Chromosome: | chromosome_1 |
Location: | 7966614 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g068012 | PPR5 | Pentatrichopeptide repeat protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCCATACACCTGCACACACGCACACACAC |
Internal bar code: | ATAGAAGCGACGCCGAGCTATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 104 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 37 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCCTTCTCTCTTCTTCCCA |
Suggested primer 2: | GATACACACATGACGACGGC |