| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146149 |
| Chromosome: | chromosome_8 |
| Location: | 1994924 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g367950 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGGGGTCGGCCTCCGCCAGCCCGCTGCCC |
| Internal bar code: | ACGGCCCAAGGTTAAAACACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 473 |
| LEAP-Seq percent confirming: | 98.3659 |
| LEAP-Seq n confirming: | 14146 |
| LEAP-Seq n nonconfirming: | 235 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATTTCATCAAGGATCGG |
| Suggested primer 2: | AAGACAGGAGGGGTTGTGTG |