Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.146160 |
Chromosome: | chromosome_16 |
Location: | 6796708 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g674515 | IDA7 | Flagellar inner dynein arm I1 intermediate chain IC140 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCAGGCGCTGCATGTGGCCACGCCCCGCGT |
Internal bar code: | ATAAGTGGAGTAGATTGATAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 300 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 32 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGGTACAGTCCTCCCTCGT |
Suggested primer 2: | TGGTGGGACCTACTACAGGC |