| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146161 |
| Chromosome: | chromosome 17 |
| Location: | 7118565 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g747397 | (1 of 1) PTHR24362//PTHR24362:SF284 - SERINE/THREONINE-PROTEIN KINASE NEK // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCTGCAGTGCTGCCGCCTGCTGTCGC |
| Internal bar code: | GAACGGAAGGCGGTACATCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 97.1429 |
| LEAP-Seq n confirming: | 272 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACGTACGACTTCGTGTC |
| Suggested primer 2: | GATGGCTTAGGGTTGTGCAT |