| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.146162 |
| Chromosome: | chromosome 2 |
| Location: | 2338236 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g090750 | (1 of 1) PF08574 - Protein of unknown function (DUF1762) (DUF1762) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCCGGCATATCTCAACTATATGTCTGC |
| Internal bar code: | CAGCCCGTCCGACTTAACACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 517 |
| LEAP-Seq percent confirming: | 99.6415 |
| LEAP-Seq n confirming: | 4725 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTAAGCGGTGTGAACTGGG |
| Suggested primer 2: | GTTGGCGTAGTAGCTCTCGG |