| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146180 |
| Chromosome: | chromosome 9 |
| Location: | 3987503 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392729 | (1 of 2) 2.1.2.9 - Methionyl-tRNA formyltransferase / transformylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACGGCATCCCAACCCCCCTCAGCCGTAC |
| Internal bar code: | CGTTAATAGGGACCGCAACCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 729 |
| LEAP-Seq percent confirming: | 99.5089 |
| LEAP-Seq n confirming: | 1621 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACCCCATTGAGATCAAGA |
| Suggested primer 2: | GTTGCAGTTTGTGTCCCCTT |