| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.146196 |
| Chromosome: | chromosome 9 |
| Location: | 1744009 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396600 | HYD2,HYDA2 | (1 of 2) 1.12.7.2 - Ferredoxin hydrogenase / Uptake hydrogenase; Iron hydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTTCGCGGCCGACCTGACCATCATGGA |
| Internal bar code: | CATCGTCGGTACCTACTATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 98.6667 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTTCTCGGACAGGTAGG |
| Suggested primer 2: | GCCACTTTCTTGCTTCGTTC |