| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.146210 |
| Chromosome: | chromosome 4 |
| Location: | 2555621 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222402 | (1 of 20) PF01822 - WSC domain (WSC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCATGCACTGCTGGGTCTAACGGTAATAA |
| Internal bar code: | CGTTAAGATACAAGGATCATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 494 |
| LEAP-Seq percent confirming: | 99.8843 |
| LEAP-Seq n confirming: | 863 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGTTTGGTTCGAAGATG |
| Suggested primer 2: | TCACCAACACTTCGCTTCAG |