| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.146229 |
| Chromosome: | chromosome 6 |
| Location: | 6427939 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292450 | PUS13 | RNA pseudouridine synthase; (1 of 1) 5.4.99.44 - Mitochondrial tRNA pseudouridine(27/28) synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGCGAGGGTGTCGACACTCCAGACGGT |
| Internal bar code: | GATTCGAAGTCTACGGGCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 232 |
| LEAP-Seq percent confirming: | 99.7423 |
| LEAP-Seq n confirming: | 6967 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGAGACAAGACCCGAAAG |
| Suggested primer 2: | AGGGCCCTCATTAGCAATTT |