Insertion junction: LMJ.RY0402.146254_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.146254
Chromosome:chromosome 3
Location:4315064
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g174850 CGL111 (1 of 1) PTHR19308:SF8 - STAR-RELATED LIPID TRANSFER PROTEIN 7, MITOCHONDRIAL; lipid-binding START protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTACTTTGACGACGACTTCCGCCCCAAATG
Internal bar code:GGACATCCGAAACAACGGGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:729
LEAP-Seq percent confirming:99.6896
LEAP-Seq n confirming:3854
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GCTACTAGCTGGGCCCTCTT
Suggested primer 2:GACGTCCATCTTCACCACCT