| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.146273 |
| Chromosome: | chromosome 3 |
| Location: | 3277875 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165900 | PGM17 | (1 of 3) 3.1.3.46 - Fructose-2,6-bisphosphate 2-phosphatase / Fructose-2,6-bisphosphatase; Putative phosphoglycerate mutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACCCCCTTATCCTGGTCGTGTGGCAGC |
| Internal bar code: | ACTACAAGTGCACCAAGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 195 |
| LEAP-Seq percent confirming: | 99.7305 |
| LEAP-Seq n confirming: | 740 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTTGGCCTCTAGTTCTG |
| Suggested primer 2: | CGCATCTTCTCTGGTGTCAA |