| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.146276 |
| Chromosome: | chromosome 1 |
| Location: | 3985672 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025900 | (1 of 7) PF00415//PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1) // Regulator of chromosome condensation (RCC1) repeat (RCC1_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATTGAACGACTGGGGGCAGCTGGGACAC |
| Internal bar code: | GCTTCCCTCAGGGTTATTTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 99.1045 |
| LEAP-Seq n confirming: | 332 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACCCTCATCAAGTGTCT |
| Suggested primer 2: | CCAAACGACCAAAGCGTTAT |