| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146287 |
| Chromosome: | chromosome 16 |
| Location: | 2368578 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659900 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATCCCCGCGCAGCTGGACAACCTGCCGA |
| Internal bar code: | AGGCACTTGAACTTTCGTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 86.2315 |
| LEAP-Seq n confirming: | 3263 |
| LEAP-Seq n nonconfirming: | 521 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGGATGGCATTGATGAG |
| Suggested primer 2: | AACATTTGCTTATCACCCGC |