| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146289 |
| Chromosome: | chromosome 16 |
| Location: | 4381609 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671400 | ARS1 | (1 of 2) IPR000917//IPR012083//IPR017850 - Sulfatase // Arylsulfatase // Alkaline-phosphatase-like, core domain; Periplasmic arylsulfatase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGGGGAATCACGCACGCGGCAGTACCA |
| Internal bar code: | TGCCTGTCGATTACAAATAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 467 |
| LEAP-Seq percent confirming: | 99.0824 |
| LEAP-Seq n confirming: | 15117 |
| LEAP-Seq n nonconfirming: | 140 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGCAGACGCTACGAAGG |
| Suggested primer 2: | ACTCCAAGTACGACCGCATC |